deeptools

Runs deepTools command-line programs on NGS alignment data: bamCoverage and bamCompare for BAM to bigWig/bedGraph with RPGC, CPM, RPKM or BPM normalization, multiBamSummary with plotCorrelation and plotPCA, plotFingerprint, computeMatrix with plotHeatmap and plotProfile, and alignmentSieve with --ATACshift. Use when converting BAM files to normalized coverage tracks. Use when checking ChIP-seq quality or comparing replicates. Use when plotting signal around TSS or peak regions. Use when comparing treatment versus control samples. Use when building ChIP-seq, RNA-seq or ATAC-seq coverage workflows. Not for peak calling or differential expression analysis.

Version

1.3

License

BSD license

Compatibility

Requires Python >3.8 and deepTools 3.5.6-compatible dependencies. The upstream project recommends conda/bioconda for full dependency resolution; repo examples use uv with pinned PyPI installs for reproducible command-line workflows.