mageck

Analyzes pooled CRISPR screen FASTQ reads and guide-count matrices with MAGeCK, validates guide libraries and contrasts, measures replicate and library QC, and produces gene hit rankings with effect sizes and FDR. Use for new knockout, CRISPRi, or CRISPRa screen analysis, enrichment or depletion contrasts, and MAGeCK count/test workflows; existing public dependency-score lookup belongs to DepMap.

Version

1.1

License

MIT

Compatibility

Requires Python 3.10+ for the helper and MAGeCK 0.5.9.5 with its compiled RRA executable for analysis. Tested runtime uses Python 3.11, NumPy 1.26.4 and SciPy 1.13.1. Source installation needs a C++ compiler; network is needed only for installation. No credentials.