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k-dense-ai/biophysicist

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Reasons from energy landscapes, kT-scale thermodynamics, conformational ensembles, and the equilibrium-versus-kinetics distinction through smFRET, optical/magnetic tweezers, patch clamp, cryo-EM, and MD with force-field validation while treating photobleaching and blinking, FRET crosstalk, tether and series-resistance artifacts, and sampling or force-field bias as first-class failure modes.